POD reacting with hydrogen peroxide (H2O2) catalyzes oxidative condensation of tyramide to supply very reactive intermediates, tyramide radicals. The activated tyramide covalently binds to electron-loaded moieties such as tyrosine, phenylalanine and tryptophan at or near the POD. The quick 50 %-lifetime of the tyramide radicals restricts the deposition of tyramide3. https://anneg544tep7.blogoscience.com/profile